3KQ4

Structure of Intrinsic Factor-Cobalamin bound to its receptor Cubilin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Collection date	2009-02-20
Wavelength(s)	0.9
Spacegroup name	C 2 2 21
Unit cell lengths	117.684, 204.179, 410.023
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.848 - 3.300
R-factor	0.2114
Rwork	0.211
R-free	0.24250
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.930
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.300
Rmerge	0.130
Number of reflections	72280
<I/σ(I)>	11.5
Completeness [%]	96.9
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1
1