3KPB
Crystal Structure of the CBS domain pair of protein MJ0100 in complex with 5 -methylthioadenosine and S-adenosyl-L-methionine.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Collection date | 2009-09-19 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.987, 49.704, 59.120 |
| Unit cell angles | 80.11, 75.85, 64.04 |
Refinement procedure
| Resolution | 57.170 - 1.600 |
| R-factor | 0.204 |
| Rwork | 0.202 |
| R-free | 0.22900 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.144 |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 57.170 |
| High resolution limit [Å] | 1.600 |
| Number of reflections | 55517 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 291 | 10% PEG 6000, 0.1 M TRIS pH 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
