3KMW

Crystal structure of the ILK/alpha-parvin core complex (MgATP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2008-12-15
Detector	RIGAKU SATURN 944+
Spacegroup name	P 1 21 1
Unit cell lengths	44.134, 117.103, 47.393
Unit cell angles	90.00, 101.96, 90.00

Refinement procedure

Resolution	46.360 - 2.000
R-factor	0.199
Rwork	0.199
R-free	0.20300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kmu
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.360	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	31165
<I/σ(I)>	12.2	7.8
Completeness [%]	98.3	98.8
Redundancy	5.53	6.09

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.8	277	0.05 M Bis-Tris, 12% PEG 5000 MME, 5% 1-propyl alcohol, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K