3KMD
Crystal structure of the p53 core domain bound to a full consensus site as a self-assembled tetramer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Collection date | 2009-02-07 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.084, 93.713, 145.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.516 - 2.150 |
| R-factor | 0.2173 |
| Rwork | 0.216 |
| R-free | 0.23950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ac0 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.034 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
| Rmerge | 0.138 | 0.085 | 0.614 |
| Number of reflections | 52544 | ||
| <I/σ(I)> | 6.8 | ||
| Completeness [%] | 99.9 | 100 | 98.7 |
| Redundancy | 7 | 6.9 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 6.68 | 291 | 16% PEG 4000, 142mM Nacl, pH 6.68, hanging drop, temperature 291K |
