3KLO

Vibrio cholerae VpsT bound to c-di-GMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0809
Spacegroup name	P 41 21 2
Unit cell lengths	121.703, 121.703, 208.227
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.862 - 2.802
R-factor	0.2483
Rwork	0.246
R-free	0.29040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kln
RMSD bond length	0.007
RMSD bond angle	1.049
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 2009_02_15_2320_3))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.970
High resolution limit [Å]	2.800	2.800
Number of reflections	60780
<I/σ(I)>	18.2	4
Completeness [%]	97.8	97.3
Redundancy	14.5	14.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.1M Tris-Cl, 0.8M potassium sodium tartrate tetrahydrate, 3-5% PEG-MME 5000, 8-12% Xylitol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K