3KGV
Crystal Structure of Human DNA-dependent Protein Kinase Catalytic Subunit (DNA-PKcs)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.2549, 1.2552 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 168.600, 132.990, 297.000 |
| Unit cell angles | 90.00, 105.58, 90.00 |
Refinement procedure
| Resolution | 100.000 - 6.600 |
| R-factor | 0.442 |
| Rwork | 0.442 |
| R-free | 0.44100 |
| Structure solution method | MAD |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX (1.24.1b) |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 6.750 |
| High resolution limit [Å] | 6.600 | 6.600 |
| Rmerge | 0.077 | 0.550 |
| Number of reflections | 22653 | |
| Completeness [%] | 97.6 | 97.3 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 292 | 8% PEG 8000, 0.2M NaCl, 0.05M DTT, 0.1M Bis-Tris, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
