3KD5
Closed ternary complex of an RB69 gp43 fingers domain mutant complexed with an acyclic GMP terminated primer template pair and phosphonoformic acid.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-08-15 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.03315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.855, 122.481, 133.371 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.690 |
| R-factor | 0.21939 |
| Rwork | 0.215 |
| R-free | 0.26317 |
| Structure solution method | isomorphous replacement |
| Starting model (for MR) | 3kd1 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.958 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.6.0041) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.113 | |
| Number of reflections | 36065 | |
| <I/σ(I)> | 26.9 | 3.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 23.4 | 21.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 5% PEG 20000, 0.1M sodium acetate pH5, 0.1M magnesium acetate, 0.1M Tris HCl pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
