3K72
Structure of integrin alphaX beta2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97928 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 160.962, 165.549, 536.178 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.475 - 3.700 |
| R-factor | 0.3152 |
| Rwork | 0.315 |
| R-free | 0.33460 |
| Structure solution method | MAD |
| RMSD bond length | 0.093 |
| RMSD bond angle | 1.179 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.860 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Rmerge | 0.104 | 1.355 |
| Number of reflections | 76217 | |
| <I/σ(I)> | 12.6 | 1.9 |
| Completeness [%] | 99.4 | 99.4 |
| Redundancy | 7.5 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 277 | 0.915M sodium/potassium phosphate, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
