3K70
Crystal structure of the complete initiation complex of RecBCD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-07-06 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 133.800, 192.900, 334.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 3.590 |
R-factor | 0.248 |
Rwork | 0.248 |
R-free | 0.29600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1w36 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.500 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.790 |
High resolution limit [Å] | 3.590 | 3.590 |
Rmerge | 0.075 | 0.351 |
Number of reflections | 97332 | |
<I/σ(I)> | 6.6 | 2.1 |
Completeness [%] | 96.7 | 97.5 |
Redundancy | 3.2 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 285 | 100 mM Hepes pH 7.0, 300 mM Calcium acetate, 6-8% PEG 20000, VAPOR DIFFUSION, HANGING DROP, temperature 285.0K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Hepes | ||
2 | 1 | 1 | Calcium acetate | ||
3 | 1 | 1 | PEG 20000 | ||
4 | 1 | 2 | Hepes | ||
5 | 1 | 2 | Calcium acetate | ||
6 | 1 | 2 | PEG 20000 |