3K5M
Crystal structure of E.coli Pol II-abasic DNA-ddGTP Lt(-2, 2) ternary complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-01-10 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.109, 95.228, 144.735 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.430 - 2.040 |
| Rwork | 0.243 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3k59 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.707 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.110 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.073 | 0.500 |
| Number of reflections | 62414 | |
| <I/σ(I)> | 15.6 | 3.1 |
| Completeness [%] | 95.8 | 98.7 |
| Redundancy | 4.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20-25% PEGMME3350, 0.1 M ammonium formate, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
