3K57

Crystal structure of E.coli Pol II-normal DNA-dATP ternary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	2006-10-12
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	80.425, 99.888, 125.745
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.080
Rwork	0.204
R-free	0.21600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1q8i
RMSD bond length	0.010
RMSD bond angle	1.487
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.150
High resolution limit [Å]	2.080	2.080
Rmerge	0.070	0.478
Number of reflections	57617
<I/σ(I)>	13.6	1.8
Completeness [%]	93.5	87
Redundancy	2.4	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	100 mM MES, 20% PEGmme5000, 0.2 M ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K