3K35
Crystal Structure of Human SIRT6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-07-30 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.388, 136.266, 89.260 |
| Unit cell angles | 90.00, 119.87, 90.00 |
Refinement procedure
| Resolution | 19.950 - 2.000 |
| R-factor | 0.2022 |
| Rwork | 0.202 |
| R-free | 0.26723 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yc5 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.506 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 100.000 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.086 |
| Number of reflections | 120514 |
| <I/σ(I)> | 9.5 |
| Completeness [%] | 97.1 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 293 | 2M NH4SO4, 2%PEG400, 0.1M Bis-Tris pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
