3IWU
Crystal structure of Y116T/I16A double mutant of 5-hydroxyisourate hydrolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.983 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.580, 66.592, 236.757 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.500 - 2.300 |
| R-factor | 0.18035 |
| Rwork | 0.177 |
| R-free | 0.24283 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2h1x |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.784 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.520 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.158 | 0.446 |
| Number of reflections | 46362 | |
| <I/σ(I)> | 9.3 | 6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.9 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 278 | 0.1M HEPES, 20% PEG10,000, pH7.5, VAPOR DIFFUSION, temperature 278K |
