3IUC
Crystal structure of the human 70kDa heat shock protein 5 (BiP/GRP78) ATPase domain in complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-02 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.212, 51.795, 94.994 |
| Unit cell angles | 98.85, 94.88, 117.61 |
Refinement procedure
| Resolution | 29.120 - 2.400 |
| R-factor | 0.19365 |
| Rwork | 0.190 |
| R-free | 0.26981 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3i33 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.588 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0035) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 35.000 |
| High resolution limit [Å] | 2.130 |
| Rmerge | 0.130 |
| Number of reflections | 41864 |
| Completeness [%] | 94.4 |
| Redundancy | 3.86 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 20% PEG 6000, 0.2M CaCl2, 0.1M NaAcetate, pH 5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
