3IRW
Structure of a c-di-GMP riboswitch from V. cholerae
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1050, 1.1054, 1.0762 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.461, 45.123, 76.573 |
| Unit cell angles | 90.00, 96.79, 90.00 |
Refinement procedure
| Resolution | 43.690 - 2.700 |
| R-factor | 0.199 |
| Rwork | 0.196 |
| R-free | 0.25100 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.953 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.072 | 0.382 |
| Number of reflections | 8935 | |
| <I/σ(I)> | 12.5 | 1.9 |
| Completeness [%] | 94.8 | 82.4 |
| Redundancy | 2.7 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 298 | 22% PEG550mme, 50 mM MES, pH 6.0, 5 mM MgSO4, 300 mM NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
