3IPM
Crystal Structure of Archaeal 20S Proteasome in Complex with the C-terminus of PAN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-12-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1159 |
Spacegroup name | P 42 2 2 |
Unit cell lengths | 166.890, 166.890, 412.080 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 89.510 - 4.000 |
R-factor | 0.25 |
Rwork | 0.249 |
R-free | 0.28400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ya7 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.200 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 89.500 | 4.220 |
High resolution limit [Å] | 4.000 | 4.000 |
Rmerge | 0.226 | 0.449 |
Number of reflections | 48850 | |
<I/σ(I)> | 2.9 | 2.7 |
Completeness [%] | 98.4 | 98.6 |
Redundancy | 3.2 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | 0.1 M MES pH6.8, 20% PEG 1500, VAPOR DIFFUSION, HANGING DROP, temperature 298K |