3IPD
Helical extension of the neuronal SNARE complex into the membrane, spacegroup I 21 21 21
Replaces: 3HD9Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-08-25 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9789 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 109.852, 215.680, 262.810 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.884 - 4.800 |
R-factor | 0.3051 |
Rwork | 0.304 |
R-free | 0.33180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3hd7 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.696 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.4_4)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 4.880 |
High resolution limit [Å] | 4.800 | 4.800 |
Number of reflections | 15646 | |
<I/σ(I)> | 11.1 | 2.1 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 7 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.8 | 293 | 16% MPD, 0.1M TRIS, 0.05M MAGNESIUM CHLORIDE, 3% SORBITOL, pH 8.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |