3IID
Crystal structure of the macro domain of human histone macroH2A1.1 in complex with ADP-ribose (form A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.973 |
| Spacegroup name | P 61 |
| Unit cell lengths | 86.260, 86.260, 51.040 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.860 - 1.900 |
| R-factor | 0.17769 |
| Rwork | 0.176 |
| R-free | 0.21467 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zr3 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.680 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.860 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 17128 | |
| <I/σ(I)> | 23.7 | 3.6 |
| Completeness [%] | 99.5 | 94.4 |
| Redundancy | 10 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 35% PEG 1000, 0.2 M K2NO3, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
