3IHJ

Human alanine aminotransferase 2 in complex with PLP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	77
Detector technology	CCD
Collection date	2008-12-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97
Spacegroup name	P 61 2 2
Unit cell lengths	92.730, 92.730, 262.870
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.730 - 2.300
R-factor	0.19189
Rwork	0.189
R-free	0.23767
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xi9
RMSD bond length	0.020
RMSD bond angle	1.761
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.360
High resolution limit [Å]	2.300	2.300
Number of reflections	30795
Completeness [%]	99.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2		0.1M citric acid, 40% PEG300, 0.1M disodium hydrogen phosphate , pH 4.2, VAPOR DIFFUSION, SITTING DROP