3IFA
Human muscle fructose-1,6-bisphosphatase E69Q mutant in complex with AMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-08-01 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.91841 |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 218.110, 234.260, 71.940 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.930 |
R-factor | 0.16847 |
Rwork | 0.168 |
R-free | 0.19890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fj6 |
RMSD bond length | 0.020 |
RMSD bond angle | 1.697 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0089) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.010 |
High resolution limit [Å] | 1.930 | 1.930 |
Rmerge | 0.059 | 0.364 |
Number of reflections | 128249 | |
<I/σ(I)> | 22 | 2.1 |
Completeness [%] | 92.7 | 57.2 |
Redundancy | 4.5 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 1.6M ammonium sulfate, 0.1M MES pH 6.5, 10% 1,4-dioxane, VAPOR DIFFUSION, HANGING DROP, temperature 292K |