3ICE
Rho transcription termination factor bound to RNA and ADP-BeF3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-14 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 1 |
Unit cell lengths | 69.230, 127.030, 127.170 |
Unit cell angles | 60.48, 90.26, 89.77 |
Refinement procedure
Resolution | 41.880 - 2.800 |
R-factor | 0.271 |
Rwork | 0.270 |
R-free | 0.29500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1PVO AND UNPUBLISHED STRUCTURE |
RMSD bond length | 0.013 |
RMSD bond angle | 0.970 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.900 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.079 | 0.342 |
Number of reflections | 87864 | |
<I/σ(I)> | 15.5 | 3.8 |
Completeness [%] | 94.6 | 66.6 |
Redundancy | 3.8 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.9 | 291 | 2.5% MPD, 50mM HEPES, 5mM Tris-HCL, 160mM sodium chloride, 1.25mM magnesium chloride, 5mM spermidine-HCL, 0.5mM TCEP, 1.25mM ADP-BeF3,, pH 7.9, MICROBATCH, temperature 291K |