3I8E
Crystal Structure of DDB1 in Complex with the H-Box Motif of WDR42A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2008-05-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0,1.005 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.138, 133.815, 183.999 |
| Unit cell angles | 90.00, 90.45, 90.00 |
Refinement procedure
| Resolution | 49.150 - 3.400 |
| R-factor | 0.264 |
| Rwork | 0.260 |
| R-free | 0.33800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b5m |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.264 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.150 | 3.488 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.142 | 0.541 |
| Number of reflections | 35597 | |
| <I/σ(I)> | 10.7 | 2.4 |
| Completeness [%] | 91.9 | 92.6 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 16% PEG 4000, 0.2M SODIUM CHLORIDE, 0.1M MES, 0.005M DTT , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
