3I4U

Crystal Structure Analysis of a helicase associated domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-08
Detector	MAR CCD 165 mm
Wavelength(s)	0.91164
Spacegroup name	P 41 21 2
Unit cell lengths	78.854, 78.854, 88.793
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.480 - 2.100
R-factor	0.19532
Rwork	0.193
R-free	0.24486
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	0.881
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.100
Total number of observations	557688
Number of reflections	31239
<I/σ(I)>	29.2
Completeness [%]	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	2uL drop (1:1); 1.7M (NH4)2SO4, 0.1M Tris/HCl, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	8	293	2uL drop (1:1); 1.7M (NH4)2SO4, 0.1M Tris/HCl, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K