3I2T

Crystal structure of the unliganded Drosophila Epidermal Growth Factor Receptor ectodomain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-02
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.91969
Spacegroup name	C 2 2 21
Unit cell lengths	74.378, 174.798, 161.653
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.680 - 2.700
R-factor	0.228
Rwork	0.224
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2a91
RMSD bond length	0.013
RMSD bond angle	1.624
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (1.3.3)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.000	45.000	2.800
High resolution limit [Å]	2.700	5.810	2.700
Rmerge	0.083	0.049	0.657
Number of reflections	29225
<I/σ(I)>	11.7
Completeness [%]	99.8	98.2	100
Redundancy	7	6.7	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	294	10% PEG 4,000, 0.1M HEPES, 5% Jeffamine M-600 (pH 7.0), 50mM KCl, 12.5% ethylene glycol, vapor diffusion, hanging drop, temperature 294K