3I2E
Crystal structure of human dimethylarginine dymethylaminohydrolase-1 (DDAH-1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-03-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.995, 47.926, 90.318 |
| Unit cell angles | 90.00, 94.16, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.030 |
| Rwork | 0.189 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jai |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.309 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR (2.5) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.400 | 2.100 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.074 | 0.225 |
| Number of reflections | 40755 | |
| <I/σ(I)> | 20.99 | |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 4.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 25% (w/v) PEG 6000, 0.1 M Tris-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
