3I1F

Gamma-subunit of the translation initiation factor 2 from S. solfataricus in complex with Gpp(CH2)p

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X12
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X12
Temperature [K]	203.3
Detector technology	CCD
Collection date	2007-06-19
Detector	MAR CCD 165 mm
Wavelength(s)	0.8148
Spacegroup name	P 31
Unit cell lengths	95.770, 95.770, 165.130
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.400 - 2.500
R-factor	0.2281
Rwork	0.226
R-free	0.26610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2pmd
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.400	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.109	0.531
Number of reflections	57747
<I/σ(I)>	7.73	2.2
Completeness [%]	98.7	98.7
Redundancy	2.83	2.78

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	295	15% natrium malonate dihydrate, 50mM glycine, 3mM CdCl2 at the protein/nucleotide molar ratio 1/10. For cryoprotection ethylene glycol was added to the reservoir solution at the final concentration of 15% (v/v), pH4.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K