3I0W
Crystal structure of Clostridium acetobutylicum 8-oxoguanine glycosylase/lyase in complex with dsDNA containing cytosine opposite to 8-oxoG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-01-09 |
Detector | MAR345 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 92.400, 92.400, 191.050 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.900 - 1.730 |
R-factor | 0.195 |
Rwork | 0.195 |
R-free | 0.21900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3f0z |
RMSD bond length | 0.005 |
RMSD bond angle | 1.100 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.900 | 1.800 |
High resolution limit [Å] | 1.730 | 1.730 |
Rmerge | 0.056 | 0.325 |
Number of reflections | 48375 | |
<I/σ(I)> | 32.2 | 4.93 |
Completeness [%] | 94.7 | 79.2 |
Redundancy | 14.2 | 11.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 277 | PEG-4000 16%, 0.1M NaAcetate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |