3I08
Crystal structure of the S1-cleaved Notch1 Negative Regulatory Region (NRR)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2008-12-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 65.868, 65.868, 322.586 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.320 - 3.200 |
| R-factor | 0.232 |
| Rwork | 0.229 |
| R-free | 0.28600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Notch1 NRR deletion (3eto) |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.734 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0093) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.000 | 3.260 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 11928 | |
| <I/σ(I)> | 9.9 | 2.3 |
| Completeness [%] | 94.4 | 97.1 |
| Redundancy | 5.4 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 0.1M NaOAc, 2.0 M NaCl, 10% glycerol, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
