3HXG
Crystal structure of Schistsome eIF4E complexed with m7GpppA and 4E-BP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-01 |
Detector | NOIR-1 |
Wavelength(s) | 1.5 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 45.560, 125.400, 37.340 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.000 - 2.100 |
R-factor | 0.2155 |
Rwork | 0.210 |
R-free | 0.26870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2v8w |
RMSD bond length | 0.009 |
RMSD bond angle | 1.222 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHASES |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 32.080 |
High resolution limit [Å] | 2.100 |
Rmerge | 0.113 |
Redundancy | 3.68 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 279 | 100mM pH 6.5 Mops, 20% PEG4K, 0.2M MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 279K |