3HVG
Structure of bace (beta secretase) in Complex with EV0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 232.550, 100.130, 64.780 |
| Unit cell angles | 90.00, 103.73, 90.00 |
Refinement procedure
| Resolution | 48.340 - 2.260 |
| R-factor | 0.22456 |
| Rwork | 0.222 |
| R-free | 0.26487 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b8l |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.154 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.340 | 2.440 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.045 | 0.347 |
| Number of reflections | 66428 | |
| <I/σ(I)> | 13.26 | 2.65 |
| Completeness [%] | 97.2 | 89.9 |
| Redundancy | 3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 293 | 10% PEG 4000, 100mM MES pH 6.0, VAPOR DIFFUSION, temperature 293K |
