3HUG
Crystal structure of Mycobacterium tuberculosis anti-sigma factor RslA in complex with -35 promoter binding domain of sigL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-01 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 83.070, 166.770, 178.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.880 - 2.350 |
| R-factor | 0.21332 |
| Rwork | 0.211 |
| R-free | 0.26014 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.384 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.720 | 2.480 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.065 | 0.480 |
| Number of reflections | 103640 | |
| <I/σ(I)> | 12.4 | 3 |
| Completeness [%] | 99.7 | |
| Redundancy | 4.2 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 1.0-1.2M Ammonium Sulphate, 0.1M Bis Tris Propane, 0.5-5% peg 8000 , pH 7.0-7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
