3HTU
Crystal structure of the human VPS25-VPS20 subcomplex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-06 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.562, 51.043, 76.987 |
| Unit cell angles | 90.00, 90.39, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.24035 |
| Rwork | 0.239 |
| R-free | 0.27261 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cuo |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.288 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 30553 | |
| <I/σ(I)> | 29.1 | 3.03 |
| Completeness [%] | 98.4 | 87.6 |
| Redundancy | 3.2 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 0.1M Tris-HCl pH 8.0, 35% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
