3HSY
High resolution structure of a dimeric GluR2 N-terminal domain (NTD)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-05-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.95294 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 82.452, 93.695, 101.466 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.533 - 1.750 |
R-factor | 0.178 |
Rwork | 0.178 |
R-free | 0.20500 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.064 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 68.840 | 1.840 |
High resolution limit [Å] | 1.750 | 1.750 |
Number of reflections | 79848 | |
<I/σ(I)> | 2.8 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 5 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 293 | 200mM ammonium phosphatre, 20% PEG3350, pH4.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |