3HPL

KcsA E71H-F103A mutant in the closed state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Detector technology	CCD
Collection date	2008-12-01
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	I 4
Unit cell lengths	156.140, 156.140, 76.292
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 3.200
Rwork	0.220
R-free	0.26910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.440
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	3.200
Number of reflections	12699
Completeness [%]	90.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	20-25% PEG 400, 50 mM magnesium acetate, 50 mM sodium acetate, pH 5.0-6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K