3HOW

Complete RNA polymerase II elongation complex III with a T-U mismatch and a frayed RNA 3'-uridine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2007-08-17
Detector	PSI PILATUS 6M
Wavelength(s)	0.9188
Spacegroup name	C 2 2 21
Unit cell lengths	222.645, 395.962, 283.476
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 3.600
Rwork	0.214
R-free	0.25400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hou
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	3.600	3.600
Number of reflections	144009
Redundancy		7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	200mM ammonium acetate, 300mM SODIUM ACETATE, 50mM Hepes pH 7.0, 4-6% PEG 6000, 5mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 295K