3HOB
Factor VIII Trp2313-His2315 segment is involved in membrane binding as shown by crystal structure of complex between factor VIII C2 domain and an inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-02 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.04 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.279, 58.988, 60.086 |
| Unit cell angles | 90.00, 110.65, 90.00 |
Refinement procedure
| Resolution | 33.390 - 2.070 |
| R-factor | 0.21966 |
| Rwork | 0.217 |
| R-free | 0.26278 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1d7p |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.454 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.084 | 0.415 |
| Number of reflections | 17899 | |
| <I/σ(I)> | 13.492 | |
| Completeness [%] | 93.1 | 63.9 |
| Redundancy | 3.1 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 30.0% PEG 2000, MME 0.15M KBr, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
