3HMX
Crystal structure of ustekinumab FAB/IL-12 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Collection date | 2002-06-04 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.092, 116.435, 182.781 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.660 - 3.000 |
| R-factor | 0.215 |
| Rwork | 0.211 |
| R-free | 0.30300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.883 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | CNX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.700 | 45.700 | 3.110 |
| High resolution limit [Å] | 3.000 | 6.460 | 3.000 |
| Rmerge | 0.128 | 0.073 | 0.494 |
| Number of reflections | 24337 | ||
| <I/σ(I)> | 24.5 | 4 | |
| Completeness [%] | 99.9 | 100 | 100 |
| Redundancy | 13.22 | 12.57 | 14.07 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
