3HLN
Crystal structure of ClpP A153C mutant with inter-heptamer disulfide bonds
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 182.299, 182.299, 476.859 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.220 - 3.200 |
| R-factor | 0.21542 |
| Rwork | 0.213 |
| R-free | 0.25274 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fzs |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.987 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.220 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 148306 | |
| <I/σ(I)> | 14.2 | 2.1 |
| Completeness [%] | 97.5 | 91.7 |
| Redundancy | 3 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 1 M 1,6-hexanediol, 0.1 M Sodium acetate pH 4.6, 10 mM CoCl2, 100 mM CaCl2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
