3HK2
Crystal structure of T. thermophilus Argonaute N478 mutant protein complexed with DNA guide strand and 19-nt RNA target strand
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-17 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 111.193, 110.884, 174.339 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.781 - 2.800 |
R-factor | 0.207 |
Rwork | 0.205 |
R-free | 0.24100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3dlh |
RMSD bond length | 0.007 |
RMSD bond angle | 1.242 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.056 | 0.487 |
Number of reflections | 53789 | |
<I/σ(I)> | 30.6 | 2.1 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 6.6 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 308 | 1.0 M succinic acid, 0.1 M Hepes, pH 7.0, 1% (w/v) polyethylene glycol monomethyl ether 2,000, VAPOR DIFFUSION, HANGING DROP, temperature 308 K |