3HJU
Crystal structure of human monoglyceride lipase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.976180 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 85.860, 127.230, 137.140 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.020 - 2.200 |
| R-factor | 0.19392 |
| Rwork | 0.192 |
| R-free | 0.22889 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | a partial/incomplete model of PDB entries obtained by other methods |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.839 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.020 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Total number of observations | 162505 | |
| Number of reflections | 37674 | |
| <I/σ(I)> | 3.86 | |
| Completeness [%] | 97.9 | 86.3 |
| Redundancy | 4.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | under oil | 277 | 35% v/v MPD, 70mM Sodium Cacodylate, pH 4-6, under oil, temperature 277K |
