3HIZ
Crystal structure of p110alpha H1047R mutant in complex with niSH2 of p85alpha
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-15 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 115.316, 120.697, 153.963 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.510 - 3.300 |
| R-factor | 0.26342 |
| Rwork | 0.260 |
| R-free | 0.33271 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2rd0 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.431 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC (5.2.0019) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 7.100 | 3.300 |
| Rmerge | 0.086 | 0.032 | 0.481 |
| Number of reflections | 32822 | ||
| <I/σ(I)> | 17.904 | ||
| Completeness [%] | 99.5 | 99.3 | 98.3 |
| Redundancy | 6.8 | 6.6 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | Na formate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
