3HIZ

Crystal structure of p110alpha H1047R mutant in complex with niSH2 of p85alpha

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform	polymer	1096	127841.6	1	UniProt (P42336) Pfam (PF02192) Pfam (PF00794) Pfam (PF00792) Pfam (PF00613) Pfam (PF00454)	Homo sapiens (human)	PI3-kinase p110 subunit alpha, PtdIns-3-kinase p110, PI3K
2	B (B)	Phosphatidylinositol 3-kinase regulatory subunit alpha	polymer	373	44262.5	1	UniProt (P27986) Pfam (PF00017) Pfam (PF16454)	Homo sapiens (human)	PI3-kinase p85 subunit alpha, PtdIns-3-kinase p85-alpha, PI3K

Sequence modifications

A: 1 - 1068 (UniProt: P42336)

PDB	External Database	Details
Met -28	-	expression tag
Ser -27	-	expression tag
Tyr -26	-	expression tag
Tyr -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
Asp -18	-	expression tag
Tyr -17	-	expression tag
Asp -16	-	expression tag
Ile -15	-	expression tag
Pro -14	-	expression tag
Thr -13	-	expression tag
Thr -12	-	expression tag
Glu -11	-	expression tag
Asn -9	-	expression tag
Leu -8	-	expression tag
Tyr -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Ala -3	-	expression tag
Met -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Arg 1047	His 1047	engineered mutation

B: 322 - 694 (UniProt: P27986)

PDB	External Database	Details
Asn 330	Asp 330	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	172104.1
All*	Total formula weight	172104.1

*Water molecules are not included.