3HIK
Structure of human Plk1-PBD in complex with PLHSpT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-09 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.200, 65.800, 104.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.770 |
| R-factor | 0.20607 |
| Rwork | 0.205 |
| R-free | 0.23737 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.783 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.630 |
| High resolution limit [Å] | 1.570 | 3.380 | 1.570 |
| Rmerge | 0.051 | 0.036 | 0.614 |
| Number of reflections | 28597 | ||
| <I/σ(I)> | 22.583 | ||
| Completeness [%] | 82.6 | 99.1 | 28.9 |
| Redundancy | 4.4 | 4.2 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 298 | 0.2 M di potassium phosphate, 20% w/v PEG 3350, hanging drop, temperature 298K |
