3HI9
The x-ray crystal structure of the first RNA recognition motif (RRM1) of the AU-rich element (ARE) binding protein HuR at 2.0 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00778 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 74.804, 74.804, 147.361 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.760 - 2.000 |
| R-factor | 0.199 |
| Rwork | 0.197 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fxl |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.361 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.080 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.096 | 0.484 |
| Number of reflections | 32767 | 3709 |
| <I/σ(I)> | 7.9 | 3.2 |
| Completeness [%] | 99.3 | 99.5 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.2 M Di-ammonium citrate, 20% w/v PEG 3350, 5% Glycerol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
