3HI7
Crystal structure of human diamine oxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-30 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.95663 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 92.506, 94.784, 196.525 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.570 - 1.799 |
| R-factor | 0.16205 |
| Rwork | 0.161 |
| R-free | 0.18890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | The starting model for molecular replacement was a partially refined model of the human diamine oxidase we had previously solved in C2221 this was solved using pdb code 2C10 as a starting model. |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.345 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.799 | 1.799 |
| Rmerge | 0.084 | 0.436 |
| Number of reflections | 158683 | |
| <I/σ(I)> | 20.034 | 3.7 |
| Completeness [%] | 99.0 | 95.1 |
| Redundancy | 6.4 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1M bis-tris propane, 20%(w/v) PEG 3350, 0.2M sodium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
