3HI1
Structure of HIV-1 gp120 (core with V3) in Complex with CD4-Binding-Site Antibody F105
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-11 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 412.403, 412.403, 83.206 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.480 - 2.900 |
| R-factor | 0.204 |
| Rwork | 0.202 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1BBD 1bbj |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.154 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.980 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.123 | 0.456 |
| Number of reflections | 48590 | |
| <I/σ(I)> | 13.1 | 1.21 |
| Redundancy | 4.4 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 293 | 16% PEG 6000, 8% 2-PROPANOL, 0.2M AMMONIUM SULFATE, 0.1M HEPES, PH 7.5, temperature 293K |
