3HHW
Complex of a vesicular stomatitis virus empty capsid with the nucleocapsid-binding domain of the phosphoprotein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-08 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.94 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 170.602, 234.520, 95.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.700 |
| R-factor | 0.26447 |
| Rwork | 0.263 |
| R-free | 0.29573 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qvj |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.957 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | COMO |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.073 | 0.419 |
| Number of reflections | 77814 | |
| <I/σ(I)> | 20.79 | |
| Completeness [%] | 73.1 | 44.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 0.8 M K/Na tartrate, 0.2 M NaCl, 0.1 M imidazole, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
