3HB1

Crystal structure of ed-eya2 complexed with Alf3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	1.
Spacegroup name	I 4
Unit cell lengths	183.941, 183.941, 119.976
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.510
R-factor	0.205
Rwork	0.200
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3geb
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.590
High resolution limit [Å]	2.510	2.510
Rmerge	0.065
Number of reflections	68962
<I/σ(I)>	35.5	5.5
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	291	2.4M NaCl, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K