3HAM
Structure of the gentamicin-APH(2")-IIa complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.700, 58.800, 81.400 |
| Unit cell angles | 90.00, 98.40, 90.00 |
Refinement procedure
| Resolution | 35.000 - 2.500 |
| R-factor | 0.21319 |
| Rwork | 0.210 |
| R-free | 0.27103 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.301 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 80.600 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.042 |
| Number of reflections | 25799 |
| <I/σ(I)> | 21.4 |
| Completeness [%] | 98.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | unbuffered 16% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
