3H90

Structural basis for the autoregulation of the zinc transporter YiiP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	105.657, 130.704, 115.757
Unit cell angles	90.00, 93.28, 90.00

Refinement procedure

Resolution	20.000 - 2.900
Rwork	0.261
R-free	0.27677
Structure solution method	MIR
RMSD bond length	0.009
RMSD bond angle	1.450
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.059	0.424
Number of reflections	65270
<I/σ(I)>	18.6	1.4
Completeness [%]	92.7	54.3
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	see reference, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6	293	see reference, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6	293	see reference, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6	293	see reference, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 293K